Journal: Materials Today Bio
Article Title: Self-assembled extracellular matrix-lipid nanoparticle composite for site-specific siRNA delivery to improve cardiac repair post-myocardial infarction
doi: 10.1016/j.mtbio.2025.102205
Figure Lengend Snippet: ECM-LNP composite characterization. (A) The ECM-RNA@LNP exhibited a meshwork microstructure under cryogenic SEM. LNPs, appearing as bright dots (white arrows), were closely attached to the ECM scaffold following EDC/NHS conjugation. (B) Conjugation of ECM and LNPs was assessed by FT-IR, which showed amplified C-N stretching and C=O stretching peaks. (C) XRD measurement indicated a peak around 40° in ECM-LNP composite. (D) ECM hydrogel precursor, ECM hydrogel, and ECM-LNP composites treated with various concentrations of EDC/NHS. The storage and loss moduli of the ECM hydrogel and ECM-LNP composite precursors at 37 °C were measured. (E) Rheometry analysis showed that the ECM-LNP composite had a lower storage modulus than the ECM hydrogel. (F) The gelation time was approximately 110 s for the ECM-LNP composite and about 60 s for the ECM. (G) LNP release from the ECM-LNP composite was examined using LNPs containing FITC-dextran. Increasing the EDC/NHS concentration decreased the FITC-dextran@LNP release rate. (H) ECM-LNP composites treated with EDC/NHS exhibited a more consistent FITC-dextran@LNPs release in 14 days. (n = 3 in panel E to H. t -test applied for panel F. ∗∗p < 0.01. Data are represented as mean ± standard deviation.)
Article Snippet: PerCP-Cyanine5.5-CD11b (M1/70) (CPY5-65055), FITC-MHC II (I-A/I-E) (FITC-65122-25UG), CoraLite Plus 750-CD206 (CL750-98031) antibodies were purchased from Proteintech (China).
Techniques: Conjugation Assay, Amplification, Concentration Assay, Standard Deviation
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